For more help or information you can visit the website of gpsbabel. Import from the most common GPS track files including GPX, TCX and NMEA log files. StylizeĬhoose different size, border color, and fill color for your tracks and waypoints. View information about individual track points or the entire track. Explore your Tracks Photolink 16Įxplore your GPS track history on different high quality maps. In addition, PhotoLinker has complete support for XMP sidecar files. PhotoLinker can read and write jpeg images, as well as most RAW file formats. PhotoLinker will even help you automatically set the Finder's file creation date to the date the photo was taken. Advanced Date ToolsĪdd dates to scanned to photos or copy dates between tags. Incorrect time on your camera? PhotoLinker makes it easy to correct all the times on your photos. PhotoLinker shows you exactly how the tags will be changed so that you can track exactly how your photos' metadata will be modified. Tags are read and written with the open source tool ExifTool. You can regroup, reorder, and rename tags to streamline your workflow. PhotoLinker lets you customize the metadata editor to show exactly the tags you want. PhotoLinker supports roughly 100 industry standard tags including the most popular like title, description, people, and keywords. Metadata Working Group (MWG) compliance ensures that your annotations are read from and written to your photos using new industry standards. After annotating with PhotoLinker, upload your photos to popular websites. Tags are written directly to the photo or its sidecar (and not a proprietary database) so that your annotations stay with the photo forever. Show off your adventures with a customizable map featuring your tracks and geotagged photos alongside. You can also export photo metadata in a number of formats.ĭevelopers: build your own exporter with Photolinker's plugin SDK. Photo and Metadata ExportersĮxport resized images that contain all of the correctly formatted metadata. Fine tune the image direction added by supported cameras (Note: iPhone 3GS and newer are supported). Got Direction?Īdd image direction to your photos and display the direction on a map. Whether your photos are geotagged using PhotoLinker or automatically geotagged using your camera, PhotoLinker lets you interact with and explore your photos on a number of different high quality maps. The compound may then be assayed in a soluble form. When the beads are exposed to high-intensity long-wave UV light, the linker is cleaved and the compound is released. Frequently, photo-labile linkers (photolinkers) are used for this purpose (13). It is used as a photolabile linker for the preparation of carboxylic acids. Although other protocols are available for controlling peptide presentation or modulus, few allow manipulation of material properties in situ and in the presence of cells down to the micrometer scale.PhotoLinker By drafnarofin1972 Follow | Public These PEG-based materials are useful for probing the dynamic influence of cell-cell and cell-material interactions on cell function in 2D or 3D. This comprehensive protocol is useful for controlling peptide presentation and substrate modulus during cell culture on or within an elastic matrix. Hydrogel degradation occurs on demand, in seconds to minutes, with user-directed light exposure. Preparation of sterile solutions for hydrogel fabrication takes hours, whereas the reaction to form the final hydrogel is complete in minutes. In this protocol, synthesis and purification of photodegradable monomers take approximately 2 weeks, but the process can be substantially shortened by purchasing the o-nitrobenzylether precursor. Degradation is readily achieved with cytocompatible wavelengths of low-intensity flood irradiation (365-420 nm) in minutes or with high-intensity laser irradiation (405 nm) in seconds. cell-material interactions can be probed in 2D or 3D by spatiotemporally controlling the gel microenvironment, which allows unique experiments to be performed to monitor cell response to changes in their niche. Hydrogels are synthesized with the photodegradable cross-linker in the presence or absence of cells, allowing direct encapsulation or seeding on surfaces. In this study, we provide coupling procedures to PEG-bis-amine to form a photodegradable cross-linker or to the fibronectin-derived peptide RGDs to form a photoreleasable tether. the materials are created by synthesizing an o-nitrobenzylether-based photodegradable monomer that can be coupled to primary amines. The cytocompatible chemistry and degradation process enable dynamic, tunable changes for applications in two-dimensional (2D) or 3D cell culture. Abstract : We describe a detailed procedure to create photolabile, polyethylene glycol (PEG)-based hydrogels and manipulate material properties in situ.
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